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Title: Induction of suppressor cells by Mycobacterium paratuberculosis antigen in inflammatory bowel disease.
Title Abreviation: Clin Exp Immunol Date of Pub: 1991 Feb
Author: Ebert EC; Bhatt BD; Liu S; Das KM;
Issue/Part/Supplement: 2 Volume Issue: 83 Pagination: 320-5
MESH Headings: Adolescence; Adult; Aged; Antigens, Bacterial (*IM); Child; Colitis, Ulcerative (ET/*IM); Concanavalin A (PD); Crohn Disease (ET/*IM); Human; Lymphocyte Transformation; Middle Age; Mycobacterium (*IM); Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; T-Lymphocytes, Suppressor-Effector (*IM);
Journal Title Code: DD7 Publication Type: JOURNAL ARTICLE
Date of Entry: 910319NEntry Month: 9105
Country: ENGLAND Index Priority: 2
Language: Eng Unique Identifier: 91130082
Unique Identifier: 91130082 ISSN: 0009-9104
Abstract: We studied the M. paratuberculosis-induced proliferation and suppressor cell generation by peripheral blood lymphocytes from patients with inflammatory bowel disease. Peripheral blood lymphocytes were separated from 33 patients with Crohn's disease, 18 with ulcerative colitis, nine with other intestinal diseases, and five with autoimmune disorders. Proliferation of peripheral blood lymphocytes from normal individuals in response to 10 micrograms/ml of M. paratuberculosis antigen was reduced by depletion of CD4+ T cells. The ability of M. paratuberculosis antigen to suppress concanavalin A-induced proliferation (expressed as a percentage suppression) was reduced by depletion of CD8+ T cells. This suppression was the same whether peripheral blood lymphocytes were from normal individuals, patients with intestinal diseases other than inflammatory bowel disease, or patients with autoimmune disorders (47 +/- 14%, 44 +/- 24%, and 30 +/- 26%, respectively). In contrast, the suppression induced by M. paratuberculosis for patients with Crohn's disease and ulcerative colitis (66 +/- 22% and 67 +/- 22%) was much greater than that for normal individuals (P less than 0.001). In particular, lymphocytes from patients with active Crohn's disease demonstrated little proliferation in response to this antigen but marked suppressor activity (79 +/- 13%). How the immunomodulatory effects of this antigen relate to the pathogenesis of the inflammatory bowel diseases remains to be determined.
Abstract By: Author
Address: Department of Medicine, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, New Brunswick 08903-0019.