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Title: Antibodies against Mycobacterium paratuberculosis in Crohn's disease.
Title Abreviation: QJM Date of Pub: 1996 Mar
Author: Walmsley RS; Ibbotson JP; Chahal H; Allan RN;
Issue/Part/Supplement: 3 Volume Issue: 89 Pagination: 217-21
MESH Headings: Antibodies, Bacterial (*BL); Celiac Disease (IM); Colitis, Ulcerative (IM); Comparative Study; Crohn Disease (*IM); Enzyme-Linked Immunosorbent Assay; Human; IgA (*BL); IgG (*BL); Mycobacterium paratuberculosis (*IM); Prospective Studies; -RN-;
Journal Title Code: B4V Publication Type: JOURNAL ARTICLE
Date of Entry: 961024NEntry Month: 9612
Country: ENGLAND Index Priority: 2
Language: Eng Unique Identifier: 96297822
Unique Identifier: 96297822 ISSN: -HEADING-
Abstract: Until recently the investigation of serological responses to mycobacteria in patients with Crohn's disease has been hindered by the considerable degree of cross-reactivity between antigens of M. paratuberculosis, and other mycobacterial subspecies. We evaluated the serological response of Crohn's disease patients to a recently identified species-specific 18 kDa protease-resistant antigen corresponding to M. paratuberculosis bacterioferritin. The 18 kDa antigen was purified from M. paratuberculosis as previously described. Serum was obtained from 40 patients with Crohn's disease, 15 with ulcerative colitis, 25 coeliac patients, and 21 normal blood donors. Antibody levels were measured by enzyme-linked immunosorbent assay (ELISA), with anti-human IgA and IgG alkaline phosphatase conjugate. Antibody titres were expressed as the dilution giving 1/3 of the plateau binding value of a standard positive serum (MT/3). Disease activity of the Crohn's disease cases was assessed using the Harvey-Bradshaw index. There was no statistically significant elevation of the mean IgG or IgA MT/3 titres of Crohn's disease patients over controls. No patients had antibody titres greater than two standard deviations above the mean control MT/3 titres, and there was no significant correlation between Crohn's disease activity and level of antibody titres. These findings make it unlikely that M. paratuberculosis is of primary pathogenic importance in Crohn's disease.
Abstract By: Author
Address: Gastroenterology Unit, Queen Elizabeth Hospital, Birmingham, UK.