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Title: Mycobacteria in the intestine of Japanese patients with inflammatory bowel disease.
Title Abreviation: Am J Gastroenterol Date of Pub: 1995 Jan
Author: Suenaga K; Yokoyama Y; Okazaki K; Yamamoto Y;
Issue/Part/Supplement: 1 Volume Issue: 90 Pagination: 76-80
MESH Headings: Adolescence; Adult; Aged; Base Sequence; Blotting, Southern; Crohn Disease (MI); DNA Probes; Female; Human; Inflammatory Bowel Diseases (*MI); Intestinal Mucosa (*MI); Japan; Male; Middle Age; Molecular Sequence Data; Mycobacterium paratuberculosis (*IP); Polymerase Chain Reaction; Support, Non-U.S. Gov't; -RN-;
Journal Title Code: 3HE Publication Type: JOURNAL ARTICLE
Date of Entry: 950126NEntry Month: 9503
Country: UNITED STATES Index Priority: 1
Language: Eng Unique Identifier: 95100208
Unique Identifier: 95100208 ISSN: 0002-9270
Abstract: OBJECTIVES: It is still controversial whether or not a mycobacterial infection may be a cause of Crohn's disease. Mycobacterium paratuberculosis may be very difficult to detect using routine culture techniques. To clarify this, we detected mycobacterial DNA in patients with inflammatory bowel disease. METHODS: IS900 sequences highly specific to M. paratuberculosis and the groEL gene encoding a conserved mycobacterial antigen were studied in colonic mucosa using polymerase chain reaction (PCR). PCR products were analyzed by Southern blot hybridization. RESULTS: IS900 sequences were detected in all (100%) of 10 patients with Crohn's disease, in 11 (61.1%) of 18 patients with ulcerative colitis, and in 14 (87.5%) of 16 control patients with noninflammatory bowel disease. All IS900 positive samples had groEL PCR products. CONCLUSIONS: Our results, on the basis of the prevalence, do not support the hypothesis that M. paratuberculosis is involved in the pathogenesis of Crohn's disease.
Abstract By: Author
Address: First Department of Internal Medicine, Kochi Medical School, Japan.