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Title: Detection of Mycobacterium paratuberculosis by polymerase chain reaction in children with Crohn's disease.
Title Abreviation: J Infect Dis Date of Pub: 1994 Feb
Author: Dell'Isola B; Poyart C; Goulet O; Mougenot JF; Sadoun-Journo E; Brousse N; Schmitz J; Ricour C; Berche P;
Issue/Part/Supplement: 2 Volume Issue: 169 Pagination: 449-51
MESH Headings: Child, Preschool; Crohn Disease (*CO/MI); DNA Primers; DNA, Bacterial (AN); Human; Mycobacterium paratuberculosis (GE); Paratuberculosis (*DI); Polymerase Chain Reaction; Support, Non-U.S. Gov't; -RN-;
Journal Title Code: IH3 Publication Type: JOURNAL ARTICLE
Date of Entry: 940323NEntry Month: 9405
Country: UNITED STATES Index Priority: 1
Language: Eng Unique Identifier: 94149339
Unique Identifier: 94149339 ISSN: 0022-1899
Abstract: Mycobacterium paratuberculosis, the causative agent of Johne's disease (a chronic enteritis in ruminants), has been suspected to be involved in Crohn's disease. In this study, polymerase chain reaction was used to detect the presence of IS900 DNA sequences specific to M. paratuberculosis genomes in biopsies and surgical resections from 53 children with various gastrointestinal diseases and disorders. IS900 sequences were found in 13 of 18 samples from patients with Crohn's disease (72%; P < .01 vs. samples from patients without Crohn's disease), in 1 of 5 with ulcerative colitis, in 2 of 6 with severe unclassified colitis, and in 7 of 24 with other gastrointestinal illnesses. These results appear to support the hypothesis that M. paratuberculosis is involved in the pathogenesis of Crohn's disease.
Abstract By: Author
Address: Laboratoires de Microbiologie and d'Anatomopathologie, Hôpital Necker-Enfants Malades, Paris, France.