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Title: Mycobacterium paratuberculosis DNA in Crohn's disease tissue.
Title Abreviation: Gut Date of Pub: 1992 Jul
Author: Sanderson JD; Moss MT; Tizard ML; Hermon-Taylor J;
Issue/Part/Supplement: 7 Volume Issue: 33 Pagination: 890-6
MESH Headings: Adult; Aged; Aged, 80 and over; Animal; Autoradiography; Base Sequence; Blotting, Southern; Colitis, Ulcerative (MI); Comparative Study; Crohn Disease (*MI); DNA, Bacterial (*AN); Female; Human; Male; Middle Age; Molecular Sequence Data; Mycobacterium paratuberculosis (*GE); Paratuberculosis (MI); Polymerase Chain Reaction; Sensitivity and Specificity; Support, Non-U.S. Gov't; -RN-;
Journal Title Code: FVT Publication Type: JOURNAL ARTICLE
Date of Entry: 920908NEntry Month: 9211
Country: ENGLAND Index Priority: 1
Language: Eng Unique Identifier: 92354955
Unique Identifier: 92354955 ISSN: 0017-5749
Abstract: Crohn's disease has long been suspected of having a mycobacterial cause. Mycobacterium paratuberculosis is a known cause of chronic enteritis in animals, including primates, but may be very difficult to detect by culture. IS900 is a multicopy genomic DNA insertion element highly specific for M paratuberculosis. A polymerase chain reaction (PCR) based on the 5' region of IS900 and capable of the specific detection of a single M paratuberculosis genome was developed. This was applied to DNA extracts of full thickness samples of intestine removed at surgery from 40 patients with Crohn's disease, 23 patients with ulcerative colitis, and 40 control patients without inflammatory bowel disease. Stringent precautions were taken that excluded contamination artefact. M paratuberculosis was identified in 26 of 40 (65%) Crohn's disease, in 1 of 23 (4.3%) ulcerative colitis, and in 5 of 40 (12.5%) control tissues. Positive samples from Crohn's disease were from both the small intestine and colon, those from control tissues were from the colon those from control tissues were from the colon only. All PCR internal control reactions were negative. The presence of M paratuberculosis in a small proportion of apparently normal colonic samples is consistent with a previously unsuspected alimentary prevalence in humans. The presence in two thirds of Crohn's disease tissues but in less than 5% of ulcerative colitis tissues is consistent with an aetiological role for M paratuberculosis in Crohn's disease.
Abstract By: Author
Address: Department of Surgery, St George's Hospital Medical School, London.