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Title: Specific detection of Mycobacterium paratuberculosis by DNA hybridisation with a fragment of the insertion element IS900.
Title Abreviation: Gut Date of Pub: 1991 Apr
Author: Moss MT; Green EP; Tizard ML; Malik ZP; Hermon-Taylor J;
Issue/Part/Supplement: 4 Volume Issue: 32 Pagination: 395-8
MESH Headings: Base Sequence; Crohn Disease (GE/MI); DNA Insertion Elements (*); DNA Probes (*DU/GE); Human; Infant, Newborn; Molecular Probe Techniques; Molecular Sequence Data; Mycobacterium (GE/*IP); Nucleic Acid Hybridization; Paratuberculosis (MI); Polymerase Chain Reaction; Support, Non-U.S. Gov't; -RN-;
Journal Title Code: FVT Publication Type: JOURNAL ARTICLE
Date of Entry: 910612NEntry Month: 9108
Country: ENGLAND Index Priority: 1
Language: Eng Unique Identifier: 91224511
Unique Identifier: 91224511 ISSN: 0017-5749
Abstract: This paper describes the evaluation of a newly developed DNA probe for Mycobacterium paratuberculosis. DNA probe PCR278 is a 278 bp fragment obtained by polymerase chain reaction (PCR) amplification of the 5'-region of IS900, an insertion element contained in the genome of M paratuberculosis. This DNA probe can specifically distinguish M paratuberculosis from a wide range of other organisms, including members of the M avium-M intracellulare complex. When used in conjunction with the PCR amplification technique DNA probe PCR278 could detect as little as 10 fg (equivalent to two genomes) starting material of M paratuberculosis genomic DNA. Use of PCR amplification assays based on IS900, for the detection of M paratuberculosis, and homologous IS elements found in disease isolates of M avium should greatly help our understanding of the role of these organisms in Crohn's disease and other chronic inflammatory disorders.
Abstract By: Author
Address: Department of Surgery, St George's Hospital Medical School, London.