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Title: Immunohistochemical examination for mycobacteria in intestinal tissues from patients with Crohn's disease [see comments]
Title Abreviation: Gastroenterology Date of Pub: 1989 Apr
Author: Kobayashi K; Blaser MJ; Brown WR;
Issue/Part/Supplement: 4 Volume Issue: 96 Pagination: 1009-15
MESH Headings: Antibodies, Monoclonal (DU); Antigens, Bacterial (AN); Crohn Disease (*MI); Human; Immunoenzyme Techniques; Intestines (MI); Mycobacterium (*IP); Support, Non-U.S. Gov't; Support, U.S. Gov't, Non-P.H.S.; -RN-;
Journal Title Code: FH3 Publication Type: JOURNAL ARTICLE
Date of Entry: 890427NEntry Month: 8907
Country: UNITED STATES Index Priority: 1
Language: Eng Unique Identifier: 89171830
Unique Identifier: 89171830 ISSN: 0016-5085
Abstract: We conducted an immunohistochemical search for mycobacteria in the intestinal tissues of patients with Crohn's disease. Tissues obtained by biopsy or surgical resection and fixed by a variety of methods (formalin, periodate-lysine-paraformaldehyde, fresh-frozen) were reacted by an immunoperoxidase method with antibodies to (a) Mycobacterium paratuberculosis strain linda, (b) M. tuberculosis, and (c) the common mycobacterial antigen, lipoarabinomannan. Each of the antibody preparations was shown capable of detecting a variety of typical and atypical mycobacteria (M. tuberculosis, M. kansasii, M. fortuitum, M. chelonei, M. paratuberculosis, and cell wall-defective as well as cell wall-intact forms of M. avium intracellulare) under conditions identical to those used for staining the patients' tissues. We did not detect mycobacteria in any of the 67 specimens from 30 patients examined. These results, in conjunction with those of our previous serologic studies, do not support the hypothesis that infection with a Mycobacterium causes Crohn's disease.
Abstract By: Author
Address: Medicine Service, Veterans Administration Medical Center, Denver, Colorado.
Last Revision Date: 900307