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Title: Mass screening of cattle sera against 14 infectious disease agents, using an ELISA system for monitoring health in livestock.
Title Abreviation: Am J Vet Res Date of Pub: 1991 Oct
Author: Behymer DE; Riemann HP; Utterback W;
Issue/Part/Supplement: 10 Volume Issue: 52 Pagination: 1699-705
MESH Headings: Age Factors; Animal; Antibodies (*BL); Antigens (DU); California (EP); Cattle; Cattle Diseases (EP/*PC); Enzyme-Linked Immunosorbent Assay (*); Mass Screening (*VE); Prevalence; Seasons; Software; Support, Non-U.S. Gov't; -RN-;
Journal Title Code: 40C Publication Type: JOURNAL ARTICLE
Date of Entry: 920214NEntry Month: 9204
Country: UNITED STATES Index Priority: 2
Language: Eng Unique Identifier: 92117372
Unique Identifier: 92117372 ISSN: 0002-9645
Abstract: Mass screening ELISA methods were developed for testing cattle serum for antibodies against 14 common livestock diseases simultaneously. The absorbance values were transformed to a %ELISA (spectrophotometric antibody end point) by a computer interfaced with a microplate reader. A histogram indicating a cutoff point and a report for the veterinarian also was generated. The computer program produced a print-out of the antibody profile for each animal tested, the antibody concentration against each disease, and a histogram (antibody profile) showing the prevalence of each disease in the herd. Serum samples were obtained from 1,953 cattle, including 880 dairy cattle from 10 herds and 1,073 beef cattle from 20 herds. These samples were obtained from June 1988 through June 1989. The highest antibody prevalence was against bluetongue virus. Of the 1,953 cattle tested, 1,223 (63%) were seropositive for bluetongue virus, including 502 (57%) of the dairy cattle and 721 (67%) beef cattle. Other antibody prevalences, in descending order, were: rotavirus (44%), Pasteurella spp (25%), Leptospira spp and Haemophilus spp (22%), Mycoplasma spp (18%), parainfluenza virus (17%), Campylobacter spp (16%), Anaplasma marginale (15%), bovine leukosis virus (13%), Brucella spp (8%), Mycobacterium paratuberculosis (8%), bovine viral diarrhea virus (3%), and infectious bovine rhinotracheitis virus (3%). Major differences in antibody prevalence between dairy and beef cattle were that only 4% of the dairy cattle were seropositive for A marginale, compared with 25% of the beef cattle, and conversely, 29% of the dairy cattle were seropositive for bovine leukosis virus, compared with 1% of the beef cattle.(ABSTRACT TRUNCATED AT 250 WORDS)
Abstract By: Author
Address: Department of Epidemiology and Preventive Medicine, School of Veterinary Medicine, University of California, Davis 95616.