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Title: Mucosal cell-mediated immunity to mycobacterial, enterobacterial and other microbial antigens in inflammatory bowel disease.
Title Abreviation: Clin Exp Immunol Date of Pub: 1992 Feb
Author: Ibbotson JP; Lowes JR; Chahal H; Gaston JS; Life P; Kumararatne DS; Sharif H; Alexander-Williams J; Allan RN;
Issue/Part/Supplement: 2 Volume Issue: 87 Pagination: 224-30
MESH Headings: Antigens, Bacterial (IM); Antigens, Fungal (IM); Antigens, Viral (IM); Candida (IM); Colitis, Ulcerative (*IM); Crohn Disease (*IM); Enterobacteriaceae (*IM); Human; Immunity, Cellular (*); Interleukin-2 (PD); Intestinal Mucosa (*IM); Lymphocyte Transformation; Mycobacterium (*IM); Orthomyxoviridae (IM); Support, Non-U.S. Gov't; -RN-;
Journal Title Code: DD7 Publication Type: JOURNAL ARTICLE
Date of Entry: 920312NEntry Month: 9205
Country: ENGLAND Index Priority: 2
Language: Eng Unique Identifier: 92136636
Unique Identifier: 92136636 ISSN: 0009-9104
Abstract: Culture studies have suggested that Mycobacterium paratuberculosis may play a role in the aetiology of Crohn's disease. However, evidence of sensitization to mycobacterial antigens amongst patients with Crohn's disease has not yet been adequately demonstrated. Previous studies of cell-mediated immunity (CMI) in Crohn's disease were restricted to responses of peripheral blood mononuclear cells (PBMC) to mycobacterial antigens. In this study we have investigated the proliferative responses of both PBMC and mesenteric lymph node mononuclear cells (MLNMC) to a range of mycobacterial and non-mycobacterial antigens. There was no evidence of specific sensitization in the responses of MLNMC and PBMC from patients with inflammatory bowel disease (IBD) to the mycobacterial antigens. However, anergy to M. paratuberculosis could not be excluded. IBD MLNMC responses to most antigens were generally greater than those of PBMC, which were often undetectable. When compared with controls, there was evidence of increased CMI to a range of non-mycobacterial antigens, especially Yersinia enterocolitica, amongst both MLNMC and PBMC from patients with Crohn's disease and ulcerative colitis (UC). These results do not provide support to the proposed role of mycobacteria in the pathogenesis of Crohn's disease, but indicate that further investigation may determine a role for bacterial-specific T cell-mediated responses in the pathogenesis of IBD.
Abstract By: Author
Address: Department of Infection, University of Birmingham, UK.