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Title: Cell-mediated immunity in malnourished guinea pigs after Mycobacterium bovis BCG vaccination.
Title Abreviation: Infect Immun Date of Pub: 1982 Mar
Author: McMurray DN; Yetley EA;
Issue/Part/Supplement: 3 Volume Issue: 35 Pagination: 909-14
MESH Headings: Animal; BCG Vaccine (*IM); Female; Guinea Pigs; Hypersensitivity, Delayed; Immunity, Cellular; Lymph Nodes (MI); Lymphocyte Transformation; Mycobacterium bovis (GD); Phytohemagglutinins (PD); Protein Deficiency (*IM); Support, Non-U.S. Gov't; Support, U.S. Gov't, P.H.S.; Tuberculin (IM); Vaccination; -RN-;
Journal Title Code: GO7 Publication Type: JOURNAL ARTICLE
Date of Entry: 820614NEntry Month: 8208
Country: UNITED STATES Index Priority: 2
Language: Eng Unique Identifier: 82166368
Unique Identifier: 82166368 ISSN: 0019-9567
Abstract: Specific-pathogen-free guinea pigs were vaccinated with viable Mycobacterium bovis BCG and maintained on purified, isocaloric diets containing either 30% or 7.5% casein, or commercial chow. At intervals of 4, 5, 6, and 8 weeks postvaccination, groups of guinea pigs from each diet treatment were skin tested with purified protein derivative and killed. Protein-deficient animals exhibited progressive reductions in total serum proteins and albumin. Significantly greater numbers of viable M. bovis BCG were recovered from the vaccination site and inguinal lymph nodes of protein-deficient guinea pigs at all intervals. In contrast, the development of delayed hypersensitivity was markedly retarded in the 7.5% casein group and was also reduced somewhat in the 30% casein group as compared to chow control. Peripheral blood lymphocytes from protein-deficient animals did not respond normally in vitro to a polyclonal T cell mitogen, phytohemagglutinin. These results demonstrate that protein-calorie malnutrition in this model impairs the development of cell-mediated immunity as evidenced by skin test anergy, lymphocyte hyporesponsiveness, and failure to control levels of viable M. bovis BCG after vaccination.
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