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Title: Molecular evidence for two forms of Crohn disease.
Title Abreviation: Proc Natl Acad Sci U S A Date of Pub: 1994 Dec 20
Author: Gilberts EC; Greenstein AJ; Katsel P; Harpaz N; Greenstein RJ;
Issue/Part/Supplement: 26 Volume Issue: 91 Pagination: 12721-4
MESH Headings: Actins (GE); Adult; Aged; Antigens, CD3 (*GE); Base Sequence; Crohn Disease (*CL/GE/PA); Cytokines (*GE); DNA Primers (CH); Female; Gene Expression; Human; Interleukin-1 (*GE); Male; Middle Age; Molecular Sequence Data; RNA, Messenger (GE); -RN-;
Journal Title Code: PV3 Publication Type: JOURNAL ARTICLE
Date of Entry: 950202NEntry Month: 9504
Country: UNITED STATES Index Priority: 2
Language: Eng Unique Identifier: 95108030
Unique Identifier: 95108030 ISSN: 0027-8424
Abstract: Recent epidemiological evidence suggests that there are two forms of Crohn disease (CD): perforating and nonperforating. We hypothesized that, just as with tuberculoid and lepromatous leprosy, differences in the two forms of CD would be both identified and determined by differences in the host immune response. Resected intestinal tissue from control patients as well as perforating and nonperforating CD patients was evaluated for mRNA levels. We employed 32P PCR amplification with published or custom-designed primers of a housekeeping gene (beta-actin); a human T-cell marker (CD3-delta); and the cytokines tumor necrosis factor alpha, transforming growth factor beta, granulocyte/macrophage colony-stimulating factor, interleukin (IL) 1 beta, IL-1ra, and IL-6. Differences were identified with IL-1 beta (control = 162 +/- 57 vs. perforating = 464 +/- 154 vs. nonperforating = 12,582 +/- 4733; P < or = 0.02) and IL-1ra (control = 1337 +/- 622 vs. perforating = 2194 +/- 775 vs. nonperforating = 9715 +/- 2988; P < or = 0.02). These data corroborate the epidemiological observation that there are two forms of CD. Nonperforating CD, the more benign form, is associated with increased IL-1 beta and IL-1ra mRNA expression. We conclude that it is the host immune response that determines which form of CD becomes manifest in any given individual and discuss the investigative, diagnostic, and therapeutic implications of these observations.
Abstract By: Author
Address: Department of Surgery, Mount Sinai School of Medicine, City University of New York, NY 10029.