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Title: Mycobacterium paratuberculosis DNA not detected in Crohn's disease tissue by fluorescent polymerase chain reaction.
Title Abreviation: Gut Date of Pub: 1995 Nov
Author: Rowbotham DS; Mapstone NP; Trejdosiewicz LK; Howdle PD; Quirke P;
Issue/Part/Supplement: 5 Volume Issue: 37 Pagination: 660-7
MESH Headings: Adolescence; Adult; Aged; Aged, 80 and over; Base Sequence; Colitis, Ulcerative (MI); Colon (MI); Crohn Disease (*MI); DNA Primers (CH); DNA, Bacterial (*IP); Female; Human; Intestine, Small (MI); Male; Middle Age; Molecular Sequence Data; Mycobacterium paratuberculosis (*IP); Polymerase Chain Reaction (*); Sensitivity and Specificity; Support, Non-U.S. Gov't; -RN-;
Journal Title Code: FVT Publication Type: JOURNAL ARTICLE
Date of Entry: 960220NEntry Month: 9604
Country: ENGLAND Index Priority: 1
Language: Eng Unique Identifier: 96148581
Unique Identifier: 96148581 ISSN: 0017-5749
Abstract: The role of mycobacteria in the aetiology of Crohn's disease has been a contentious subject for many years. Mycobacterium paratuberculosis is known to cause a chronic granulomatous enteritis in animals (Johne's disease) and has been implicated as a possible infectious cause of Crohn's disease. However this fastidious organism is only rarely detected by conventional microbiological techniques. This study used oligonucleotide primers to the species-specific M paratuberculosis IS900 DNA insertion element and the polymerase chain reaction to amplify any M paratuberculosis DNA from intestinal tissue DNA extracts. One oligonucleotide primer was fluorochrome-labelled and the presence of fluorescent amplified product was determined using an automated DNA sequencer with a computerised gel-scanning laser. This method was shown capable of detecting 1-2 mycobacterial genomes. Intestinal tissue samples were obtained from 68 patients with histologically confirmed Crohn's disease, 49 patients with histologically confirmed ulcerative colitis, and 26 non-inflammatory bowel disease controls. In no case was M paratuberculosis detected in any of the inflammatory bowel disease tissue samples and only one non-inflammatory bowel disease case was positive. These results do not support the hypothesis that M paratuberculosis has an aetiological role in Crohn's disease.
Abstract By: Author
Address: Division of Medicine, St James's University Hospital, Leeds.