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Title: Mycobacterium paratuberculosis and inflammatory bowel disease: frequency distribution in serial colonoscopic biopsies using the polymerase chain reaction.
Title Abreviation: Microbios Date of Pub: 1995
Author: Murray A; Oliaro J; Schlup MM; Chadwick VS;
Issue/Part/Supplement: 337 Volume Issue: 83 Pagination: 217-28
MESH Headings: Animal; Base Sequence; Biopsy; Colitis, Ulcerative (MI/PA); Colonoscopy; Crohn Disease (MI/PA); DNA, Bacterial (AN); Female; Human; Inflammatory Bowel Diseases (*MI/PA); Molecular Sequence Data; Mycobacterium paratuberculosis (GE/IP/*PH); Mycobacterium Infections (*CO); Polymerase Chain Reaction; Sensitivity and Specificity; Support, Non-U.S. Gov't; -RN-;
Journal Title Code: MXS Publication Type: JOURNAL ARTICLE
Date of Entry: 960314NEntry Month: 9605
Country: ENGLAND Index Priority: 2
Language: Eng Unique Identifier: 96147597
Unique Identifier: 96147597 ISSN: 0026-2633
Abstract: An association between Mycobacterium paratuberculosis and Crohn's disease is suspected but the evidence remains controversial. Using a one-step DNA extraction procedure with the thermophilic protease PRETAQ and amplification by the polymerase chain reaction, M. paratuberculosis DNA was detected in 22% of patients with Crohn's disease, and in 13% of patients with ulcerative colitis. M. paratuberculosis DNA was not found in any biopsy tissue from control non-inflammatory bowel disease patients. The biopsy tissues in which M. paratuberculosis was detected all came from regions which were inflamed when viewed microscopically. Overall, 7.7% of biopsies from such inflamed areas were positive. This low frequency of detection could be explained on the basis of extremely low abundance of the organism in relation to the area of mucosa sampled, or be consistent with a non-aetiological role for M. paratuberculosis in inflammatory bowel disease.
Abstract By: Author
Address: Department of Veterinary Pathology and Public Health, Massey University, Palmerston North, New Zealand.