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Title: Mycobacterium paratuberculosis in intestinal tissue from patients with Crohn's disease demonstrated by a nested primer polymerase chain reaction.
Title Abreviation: Scand J Gastroenterol Date of Pub: 1994 Oct
Author: Lisby G; Andersen J; Engbaek K; Binder V;
Issue/Part/Supplement: 10 Volume Issue: 29 Pagination: 923-9
MESH Headings: Adolescence; Adult; Aged; Aged, 80 and over; Base Sequence; Colitis, Ulcerative (DT/MI); Colon (*MI); Colonic Diseases (DT/MI); Crohn Disease (DT/*MI); DNA, Bacterial (*AN); Female; Human; Ileum (*MI); Male; Middle Age; Molecular Sequence Data; Mycobacterium paratuberculosis (GE/*IP); Paraffin Embedding; Polymerase Chain Reaction (*); Prednisolone (TU); Support, Non-U.S. Gov't; -RN-;
Journal Title Code: UCS Publication Type: CLINICAL TRIAL
Date of Entry: 950224NEntry Month: 9505
Country: NORWAY Index Priority: 2
Language: Eng Unique Identifier: 95141026
Unique Identifier: 95141026 ISSN: 0036-5521
Abstract: BACKGROUND: The etiology of Crohn's disease remains unknown, but current research has concentrated on autoimmunity and/or mycobacterial infection. The polymerase chain reaction (PCR) enables the detection of genetic material even when very few microorganisms are present. METHODS: A nested primer PCR for detection of a multi-copy insertional element (IS900) specific for Mycobacterium paratuberculosis was applied to DNA extracted from fresh and from paraffin-embedded intestinal tissue obtained from patients undergoing surgery. RESULTS: In fresh intestinal tissue from 11 of 24 patients with Crohn's disease, from 2 of 10 patients with ulcerative colitis, and from 3 of 28 patients with other colonic disorders, specific M. paratuberculosis DNA was found. In paraffin-embedded Crohn's disease tissue the presence of specific M. paratuberculosis DNA was also increased. CONCLUSIONS: Whether the presence of M. paratuberculosis is connected to the inflammatory bowel disease or is a mere coincidence cannot be stated. We find this presence interesting and encouraging for further investigations.
Abstract By: Author
Address: Dept. of Clinical Microbiology, University of Copenhagen, Herlev Hospital, Denmark.